BPXTM qPLEX Wound Profile Version 2

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KWO-96U2-01032024

KWOU2-06112024

 

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Specifications

Gram-Positive Bacteria:

  1. Staphylococcus aureus
  2. Enterococcus faecalis
  3. Staphylococcus epidermidis
  4. Enterococcus faecium

Gram-Negative Bacteria:

  1. Acinetobacter baumannii
  2. Escherichia coli
  3. Enterobacter cloacae
  4. Pseudomonas aeruginosa
  5. Klebsiella oxytoca
  6. Klebsiella pneumoniae
  7. Proteus vulgaris
  8. Citrobacter freundii
  9. Morganella morganii
  10. Proteus mirabilis
  11. Klebsiella aerogenes
  12. Bacteroides fragilis
  13. Kingella kingae

Clostridia:

  1. Clostridium novyi
  2. Clostridium septicum
  3. Clostridium perfringens

Streptococci:

  1. Group A Strep
  2. Group B Strep
  3. Group C & G Strep

Internal Control:

  1. RNase P

AmpC Resistance Marker:

  1. ampC

Methicillin Resistance Marker:

  1. mecA
  2. femA

Quinolone and fluoroquinolone Resistance Marker:

  1. QnrB
  2. QnrA

Vanomycin Resistance Marker:

  1. vanA1
  2. vanA2
  3. vanB

Carbapenem Resistance Marker:

  1. NDM
  2. KPC
  3. VIM/IMP-7
  4. OXA-48

ESBL Resistance Marker:

  1. SHV
  2. TEM
  3. CTX-M group 1
  4. CTX-M group 2

Trimethoprim Resistance Marker:

  1. DfrA
  2. DfrA1
  3. DfrA5

Macrolide Resistance Marker

  1. mefA
  2. ErmA
  3. ErmB

Tetracycline Resistance Marker:

  1. TetO
  2. TetM
  3. TetB

Aminoglycoside Resistance Marker:

  1. Antla
  2. aph3

Quinolone Resistance Marker:

  1. GyrA
  2. ParC

Bactrim Resistance Marker:

  1. Sul1
  2. Sul2

Gentamicin Resistance Marker:

  1. aph2
  2. ant2


For Research Use only. Not for use in diagnostic procedures

Kit Components:

Primer and Probe Set
Multiplex Master Mix
Positive Control
User Manual with detailed protocols and troubleshooting guide

Quality Control:
Each lot is tested for specificity, sensitivity, and reproducibility. Certificate of Analysis is available for download with each kit.

Applications:
Suitable for multiplex PCR applications. Compatible with a variety of sample types, including genomic DNA from various sources.

Storage and Stability:
Store all components at -20°C upon receipt.
Stability: 12 months from the date of manufacture when stored properly

Primer and Probe Set:
Primer Sequences: Unique and optimized for target gene amplification
Probe Design:Taqman labeled probes for increased specificity
Concentration:Pre-optimized for efficient multiplex PCR
Compatibility:Designed for use with various PCR instruments capable of 4+ channels
Purity:High purity to minimize nonspecific amplification
Format:Vials, wet
Storage:Store at -20°C for long-term stability
  
Multiplex Master Mix:
Passive Reference Dye:None
Optimization:Balanced formulation for simultaneous amplification of multiple targets
Sensitivity:Detects low-copy targets with high efficiency
Amplification Range:Broad dynamic range for reliable results
Compatibility:Suitable for standard thermal cyclers and real-time PCR instruments
Storage:Store at -20°C for long-term stability
  
Positive Control:
Source:Purified genomic DNA or synthetic DNA fragment
Concentration:Optimized for use as a positive control
Quality:Fully characterized to ensure reliability
Verification:Sequence-confirmed for target specificity
Format:Provided in a stable solution
Storage:Store at -20°C for long-term stability

 

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