BPXTM qPLEX Antibiotic Resistance Profile Version 2

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Specifications

AmpC Resistance Marker:

  1. ampC

Methicillin Resistance Marker:

  1. mecA
  2. femA

Quinolone and fluoroquinolone Resistance Marker:

  1. QnrB
  2. QnrA

Vanomycin Resistance Marker:

  1. vanA1
  2. vanA2
  3. vanB

Carbapenem Resistance Marker:

  1. NDM
  2. KPC
  3. VIM/IMP-7
  4. OXA-48

ESBL Resistance Marker:

  1. SHV
  2. TEM
  3. CTX-M group 1
  4. CTX-M group 2

Trimethoprim Resistance Marker:

  1. DfrA
  2. DfrA1
  3. DfrA5

Macrolide Resistance Marker

  1. mefA
  2. ErmA
  3. ErmB

Tetracycline Resistance Marker:

  1. TetO
  2. TetM
  3. TetB

Aminoglycoside Resistance Marker:

  1. Antla
  2. aph3

Quinolone Resistance Marker:

  1. GyrA
  2. ParC

Bactrim Resistance Marker:

  1. Sul1
  2. Sul2

Gentamicin Resistance Marker:

  1. aph2
  2. ant2
 
 

For Research Use only. Not for use in diagnostic procedures

Kit Components:
Primer and Probe Set
Multiplex Master Mix
Positive Control
User Manual with detailed protocols and troubleshooting guide

Quality Control:
Each lot is tested for specificity, sensitivity, and reproducibility. Certificate of Analysis is available for download with each kit.

Applications:
Suitable for multiplex PCR applications. Compatible with a variety of sample types, including genomic DNA from various sources.

Storage and Stability:
Store all components at -20°C upon receipt.
Stability: 12 months from the date of manufacture when stored properly

Primer and Probe Set:
Primer Sequences: Unique and optimized for target gene amplification
Probe Design:Taqman labeled probes for increased specificity
Concentration:Pre-optimized for efficient multiplex PCR
Compatibility:Designed for use with various PCR instruments capable of 4+ channels
Purity:High purity to minimize nonspecific amplification
Format:Vials, wet
Storage:Store at -20°C for long-term stability
  
Multiplex Master Mix:
Passive Reference Dye:None
Optimization:Balanced formulation for simultaneous amplification of multiple targets
Sensitivity:Detects low-copy targets with high efficiency
Amplification Range:Broad dynamic range for reliable results
Compatibility:Suitable for standard thermal cyclers and real-time PCR instruments
Storage:Store at -20°C for long-term stability
  
Positive Control:
Source:Purified genomic DNA or synthetic DNA fragment
Concentration:Optimized for use as a positive control
Quality:Fully characterized to ensure reliability
Verification:Sequence-confirmed for target specificity
Format:Provided in a stable solution
Storage:Store at -20°C for long-term stability

 

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